Expell™ displacers coupled with displacement chromatography enable significant improvements in protein purity and yield in laboratory and large scale production. Expell™ SP is SACHEM’s family of displacers for cation exchange chromatography for use in these applications. They are selectively designed to have the necessary affinity towards cation exchange chromatography media and can be predictably scaled up from lab to production scale. Regeneration protocols are provided to effectively and reproducibly remove the displacers from the chromatography column.
Advantages of using Expell™ SP displacers in high loading displacement mode:
- Protein is displaced off column at high concentration and high purity
- Purified protein contains only loading buffer allowing users to proceed directly to the next step with no additional processing
- Only the last few fractions of the final component contain Expell™ SP Due to Expell’s™ low molecular weight, it can be readily removed via dialysis or ultra filtration
- This is a reproducible process that scales up reliably
To help you get started and learn more about displacement chromatography, SACHEM’s Teaching Tools provide example results, a brief tutorial on displacement chromatography and a list of selected publications.
For your convenience, SACHEM’s Regenerate™ reagents are designed to work with Expell™ displacers for column regeneration.